Interferon-gamma (IFN-gamma ) is a secreted protein which belongs to the type II interferon family. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. Interferon-gamma is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway.
90 % as determined by SDS-PAGE, 90 % as determined by HPLC.
Form:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Target:
IFN-gamma
Application Dilute:
Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Application Notes:
Cross-Reactivity: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: Cytokines & Cytokine receptors,Cell Culture related
Recombinant Mouse IFN-gamma Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Recombinant Mouse IFN-gamma Protein is greater than 90% as determined by SEC-HPLC.
Immobilized recombinant Mouse IFNGR1 at 0.2µg/mL (100µL/well) can bind recombinant Mouse IFNG,the EC<,sub>,50<,/sub>, of Mouse IFNG is 2.2ng/mL.
M0 macrophages(Catalog RP01216S) can be induced differentiation into M1 macrophages(CD86+CD206-) by Recombinant Mouse IFN-gamma Protein (50ng/mL) and LPS (100ng/mL).
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