Key Features & Benefits:

• Complete high-throughput ATAC-Seq solution from sample through tagmentation, amplification, and library purification
• Delivers quality ATAC-Seq performance in a high-throughput workflow
• Compatible with isolated nuclei, cells or tissue samples

 

The Active Motif High-Throughput ATAC-Seq Express Kit is designed to deliver rapid, reproducible, and scalable chromatin accessibility profiling in a true 96-reaction, high-throughput format. Engineered for modern epigenomics workflows, this kit enables efficient library preparation with minimal hands-on time while maintaining the data quality and consistency required for high-impact research.

This kit integrates Pre-Indexed Assembled Tn5 Transposomes (Cat. No. ACM-53152) with the ATAC Buffer Set (Cat. No. ACM-53153), providing a fully optimized system for streamlined transposition and library generation. Pre-indexed transposomes eliminate the need for custom adapter loading and post-tagmentation indexing steps, enabling direct compatibility with multiplexed sequencing workflows and automated liquid handling platforms.

Q5 Polymerase and Library Purification (Cat. No. ACM-53159) is included to make High-Throughput ATAC-Seq Express a truly complete high-throughput ATAC-Seq solution for 96 cell or tissue samples including essential reagents for sample preparation, tagmentation, amplification, and library purification in a high-throughput-friendly protocol without sacrificing data quality.

The High-Throughput ATAC-Seq Express Kit is purpose-built for 96-well processing, utilizing the same principles as the ATAC-Seq Express Kit (Cat. No. ACM-53157) and incorporating the power of the Pre-Indexed Assembled Tn5 Transposomes 96-well breakaway plate, eliminating separate pipetting steps for incorporating indexes, making it fully compatible with multichannel pipetting and robotic automation. The standardized format minimizes variability across samples, supports high sample throughput, and simplifies experimental setup—making it ideal for large-scale studies, screening applications, and core facility environments.

By combining speed, scalability, and performance in a single integrated workflow, the Active Motif High-Throughput ATAC-Seq Express Kit empowers researchers to generate high-quality, sequencing-ready ATAC-Seq libraries with confidence, consistency, and efficiency.

 

High-Throughput ATAC-Seq Express Kit Workflow

 

High-Throughput ATAC-Seq Express Kit Contents

• Pre-indexed Assembled Tn5 Transposomes, store at -20°C
• P5 Primer (25 µM), store at -20°C
• P7 Primer (25 µM), store at -20°C
• ATAC Lysis Buffer, store at RT
• 2X Tagmentation Buffer, store at -20°C
• 1% Digitonin, store at -20°C
• 5X Q5 Reaction Buffer, store at -20°C
• dNTP Mix (10 mM each), store at -20°C
• Q5 DNA Polymerase, store at -20°C
• SPRI Beads, store at 4°C
• Silica Beads, store at 4°C
• DNA Purification Binding Buffer, store at RT
• DNA Purification Elution Buffer, store at RT
• DNA Purification Wash Buffer, store at RT