Product Description
NOTE: It is important to perform the click-reaction with Azide-x first, prior to antibody conjugation. UV illumination will damage the DBCO activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Copper-free Click Chemistry oYo-Link DBCO allows for the site-specific labeling of oYo-Link-compatible antibodies with up to two dibenzocyclooctyne (DBCO) labels per antibody for subsequent copper-free click chemistry reactions. oYo-Link-DBCO limits the attachment of the DBCO labels to the heavy chains of the antibody and therefore ensures that the DBCO label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 DBCO labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link -DBCO with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table) . Requirements: To label your compatible antibodies with oYo-Link, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list here or purchase AlphaTheras LED Photo-Crosslinking Device Here. Purchasing Information: For Credit Card purchases, proceed with checkout on website. To Request a Quote email sales alphathera.com Technical Support: For questions concerning compatibility or click-chemistry procedure email info[at]biozol.de
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