DBCO-PEG4-Biotin is a biotin probe that enables the possibility for copper-free click chemistry, which is mostly used in reaction where cytotoxic copper catalyst is not an option as live cell surface glycans or lipid studies. The click reaction takes place via strain-promoted Alkyne-Azide Chemistry (SPAAC) which does not need a Cu (I) catalysis or heating. Once the biotin is attached to a biomolecule, fluorescence microscopy detection can be carried out using the well-known signal enhancement strategy of incubation with a fluorescent streptavidin-conjugate. The introduction of the hydrophilic PEG4-moity (19 atoms, 22.8 A) reduces aggregation and precipitation during the labeling reaction and also enhances the detection efficiency of the biotin due to reducing sterically hindrance.
Molecular Weight:
749.9 g/mol
Purity:
95% (HPLC)
Form:
Solid
CAS Number:
[1255942-07-4]
Formula:
C39H51N5O8S
Application Notes:
Fluorescent labeling via click chemistry
DBCO-PEG4-Biotin
DBCO-PEG4-Biotin
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