5-Ethynyl-UTP (5-EUTP), CAS [[1193451-16-9 sodium salt 1004955-96-7 free acid]]
Biozol Catalog Number:
BCL-BCT-07-S
Supplier Catalog Number:
BCT-07-S
Alternative Catalog Number:
BCL-BCT-07-S
Manufacturer:
baseclick
Category:
Biochemikalien
Alternative Names:
Modified Triphosphate for labeling of 3END RNA or ssDNA
5-Ethynyl-UTP (5-EUTP) can be incorporated into RNA using standard nucleotide mixtures and T7 RNA polymerase in vitro transcription. For a partial modified RNA we recommend to replace the natural base by only 1-10%. Modified mRNA is ready for labeling with marker azides (e.g. fluorescent dyes for tracking and imaging or cross-linking to other biomolecules) by click chemistry.
Concentration:
100 mM
Molecular Weight:
507.97 g/mol
Purity:
98% (HPLC)
Form:
Liquid
CAS Number:
[1193451-16-9 sodium salt 1004955-96-7 free acid]
Formula:
C11H15N2O15P3
Application Notes:
During in vitro transcription, RNA polymerases efficiently incorporate 5-EUTP into RNA, introducing reactive alkyne group that enables selective post transcriptional tagging using bioorthogonal click chemistry, particularly copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC).
5-Ethynyl-UTP (5-EUTP)
5-Ethynyl-UTP (5-EUTP)
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