Full length SARS Cov-2 spike protein

Full length SARS-CoV-2 Spike Protein is single span transmembrane protein which contains the Spike protein amino acids 1-1273. Full length SARS-CoV-2 Spike Protein is manufactured in HEK293 mammalian cells, ensuring the most authentic post-translational modifications.

• Single span transmembrane membrane protein (aa 1 – 1273)
• Furin cleavage site RRAR mutated to GSAS
• Trimerization (3 x 142 kDa)
• Expressed in HEK293 Expi cells
• C-terminal Rho1D4 tag for affinity purification
• Solubilization and stabilization in LMNG detergent
• 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent

 

Species: 2019-nCoV, COVID-19.
Sequence: Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence.
Accession No.: YP_009724390.1 (GenBank), P0DTC2 (UniProt).
Tag: C-terminal Rho1D4 tag for affinity purification.
Host: Expressed in HEK293 Expi cells.
Applications: ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and Biophysical analysis.
Purity: > 98% by SDS-PAGE gel.
Trimerisation: 3 x 142 kDa. Includes transmembrane domain, a detergent screen was performed.
Purification: 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent. Confirmed by SDS-PAGE, native gel, Western Blot.
Formulation: Liquid – Purified protein (1 mg/ml) supplied in 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG.

 

For Research Use Only.