Product Description
The MB20-11 monoclonal antibody reacts with mouse CD20. CD20 is a B cell-specific 33-37 kDa transmembrane protein which is also known as B-lymphocyte antigen, B1, and Bp35. CD20 is expressed on the surface of immature and mature B cells and their malignant counterparts. CD20 plays roles in intracellular calcium regulation and B cell activation and is critical for an optimal B cell immune response against T-independent antigens. B cell depleting antibodies are valuable tools for studying B cell biology and developing therapies for autoimmune diseases and malignancies. The MB20-11 antibody rapidly and effectively depletes B cells via antibody-dependent cellular cytotoxicity (ADCC) through engaging Fcgamma receptors on monocytes and other effector cells. Isotype-specific interactions contribute significantly to the effectiveness of CD20 mAbs in vivo, with IgG2a/c mAbs having greater potency than IgG1 or IgG2b. IgG2a and IgG2c isotypes are indistinguishable in their specificities to murine FcgammaR. Mouse strains such as C57BL/6, C57BL/10, SJL, and NOD mice possesses the Igh1-b allele resulting in only the expression of IgG2c. However, mouse strains such as BALB/c and Swiss Webster mice possess the Igh1-a allele which results in only the expression of IgG2a. It is important to consider matching the Ig-haplotype of the receiving mice to the isotype of the injected antibody to avoid eliciting an undesired immune response. Bio X Cell is pleased to also offer recombinant MB20-11-CP062. This monoclonal antibody has variable domain sequences identical to MB20-11 but the constant region has been converted from mouse IgG2c to mouse IgG2a for use in mice with the Igh-1a allele. Additionally, the highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. 1: Uchida, Junji et al. ''The innate mononuclear phagocyte network depletes B lymphocytes through Fc receptor-dependent mechanisms during anti-CD20 antibody immunotherapy. The Journal of experimental medicine vol. 199,12 (2004): 1659-69. doi:10.1084/jem.20040119 2: Xiu, Yan et al. ''B lymphocyte depletion by CD20 monoclonal antibody prevents diabetes in nonobese diabetic mice despite isotype-specific differences in Fc gamma R effector functions. Journal of immunology (Baltimore, Md. : 1950) vol. 180,5 (2008): 2863-75. doi:10.4049/jimmunol.180.5.2863 3: Hamaguchi, Yasuhito et al. ''Antibody isotype-specific engagement of Fcgamma receptors regulates B lymphocyte depletion during CD20 immunotherapy. The Journal of experimental medicine vol. 203,3 (2006): 743-53. doi:10.1084/jem.20052283 4: Zhang, Zhiping et al. ''Possible allelic structure of IgG2a and IgG2c in mice. Molecular immunology vol. 50,3 (2012): 169-71. doi:10.1016/j.molimm.2011.11.006
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