Product Description
The 2.4G2 monoclonal antibody reacts specifically with mouse CD16 (FcgammaRIII) and CD32 (FcgammaRII). It has also been reported to react non-specifically via its Fc domain to FcgammaRI. CD16 and CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. These receptors bind to the Fc portion of antibody-antigen complexes and play a role in adaptive immune responses. The 2.4G2 antibody is commonly used in flow cytometry and immunofluorescence staining experiments to prevent non-specific binding of the Fc portion of IgG to the FcgammaIII and FcgammaII, and possibly FcgammaI, receptors prior to staining with antigen specific primary antibodies. The complete antibody and Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo. Note that when 2.4G2 is used for Fc blocking in immunoassays and an anti-IgG secondary-step is necessary, the secondary antibody must not be anti-rat IgG2b.
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