Klotho Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Klotho Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB101064
• Supplier Catalog Number: orb101064
• Alternative Catalog Number: BYT-ORB101064-100,BYT-ORB101064-200,BYT-ORB101064-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Klotho (401-500/1012aa)
• Conjugation: Unconjugated
• Alternative Names: KL antibody KLOT_HUMAN, Klotho peptide.

Product Description

Klotho Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 113 kDa
• UniProt: Q9UEF7
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: KL

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Klotho) Polyclonal Antibody, Unconjugated (orb101064) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Klotho) Polyclonal Antibody, Unconjugated (orb101064) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Klotho) Polyclonal Antibody, Unconjugated (orb101064) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Klotho) Polyclonal Antibody, Unconjugated (orb101064) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Klotho) Polyclonal Antibody, Unconjugated (orb101064) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human HL-60 cell lysates, Primary: Anti-Klotho (orb101064) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113 kDa, Observed band size: 130 kDa.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Klotho) Polyclonal Antibody, Unconjugated (orb101064) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Klotho) Polyclonal Antibody, Unconjugated (orb101064) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Klotho) Polyclonal Antibody, Unconjugated (orb101064) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.