SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant), Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1238673
Article Name: SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant), Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1238673
Supplier Catalog Number: orb1238673
Alternative Catalog Number: BYT-ORB1238673-0.02,BYT-ORB1238673-0.1
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Virus
Immunogen: Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H Antibody (orb1238673) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 UK variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 UK variant (B.1.1.7) Spike protein.
Conjugation: Unconjugated
Alternative Names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, sars-cov-2
SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant)
Clonality: Polyclonal
Concentration: 1 mg/mL
NCBI: 43416
UniProt: P0DTC2
Buffer: SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide.
Form: Liquid
Target: S
ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 µg/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 UK Variant Spike antibody, orb1238673, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (orb1231589).
WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7). Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1238673, 1 µg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (orb1231589).
ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and UK variant (B.1.1.7), 1 µg/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1239981, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. Immunogen region of antibody orb1239981 includes site 681P that was mutated in B.1.1.7. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 peptide (681H), but not WT peptide (681P).