SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9], IgG2b, Unconjugated, Mouse, Monoclonal
Catalog Number:
BYT-ORB1238713
Product Overview
Article Name:
SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9], IgG2b, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:
BYT-ORB1238713
Supplier Catalog Number:
orb1238713
Alternative Catalog Number:
BYT-ORB1238713-0.02,BYT-ORB1238713-0.1
Manufacturer:
Biorbyt
Host:
Mouse
Category:
Antikörper
Application:
ELISA, WB
Species Reactivity:
Virus
Immunogen:
Anti-SARS-CoV-2 Spike 26P antibody (orb1238713) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Spike protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 Spike protein.
Conjugation:
Unconjugated
Alternative Names:
SARS-CoV-2 Spike 156-157EF antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2
Product Description
SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9]
Product Properties
Clonality:
Monoclonal
Concentration:
1 mg/mL
Clone Designation:
[1C3H9]
Isotype:
IgG2b
NCBI:
43416
UniProt:
P0DTC2
Buffer:
SARS-CoV-2 Spike 26P antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
S
Images
SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA.Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 µg/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, orb1238713, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr.
ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein.Coating Antigen: SARS-CoV-2 spike S1 proteins of WT and Gamma variant (P.1), 1 µg/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, orb1238713, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (orb1231589), but not Gamma variant spike S1 protein.
ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein.Loading: 50 ng of SARS-CoV-2 spike S1 proteins, WT and Gamma variant (P.1). Detection Antibodies: Spike 26P antibody, orb1238713, 1 µg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (orb1231589), but not Gamma variant spike S1 protein.
ELISA Validation of Spike 26P Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (orb1231703) and Gamma variant (P.1) (orb1231659), 1 µg/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, orb1238713, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 26P antibody detects WT peptide (26P, orb1231703), but not Gamma variant spike S1 peptide (26S, orb1231659).
