Anti-SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) (orb1239026) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) Spike S1 glycoprotein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19) Spike S1 protein.
SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
S
ELISA Validation of SARS-CoV-2 Spike Antibody Pair.A sandwich ELISA was performed using the anti-SARS-COV-2 Spike S1 antibodies orb1239981 (10 µg/mL) as the capture antibody. Biotin-labled anti-SARS-COV-2 Spike S1 antibodies orb1239026 (1 µg/mL) and streptavidin-HRP (0.1 µg/mL) were used for detection. Detection range is from 0.8 ng to 200 ng (SARS-CoV-2 Spike S1 Protein, orb1227612).
Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, orb1274178 and orb1239026. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (orb1274178, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (orb1227612), and the anti-SARS-CoV-2 Spike S1 antibody (orb1239026, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 5.1 ng
Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, orb1274177 and orb1239026. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (orb1274177, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (orb1227612), and the anti-SARS-CoV-2 Spike S1 antibody (orb1239026, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng
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