Chemical structure and design of FCR1, showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation.
Fluorescence behavior of FCR1 in the oxidized (green) and reduced (blue) forms, using 10 µM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm.
Two photon - confocal microscopy imaging of HeLa cells treated with FCR1 (10 µM, 15 min, lambdaex = 820 nm) and (a) N-acetyl cysteine (50 µM, 30 min), (b) vehicle control and (c) H2O2 (50 µM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 µm.
Flow cytometric studies of HeLa cells treated with FCR1 (10 µM, lambdaex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red).
Fluorescence lifetimes of the donor (420 - 470 nm) in HeLa cells treated with FCR1 (10 µM, lambdaex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 µm.
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