E Cadherin (CDH1) Antibody (N-term), Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: E Cadherin (CDH1) Antibody (N-term), Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB1935497
• Supplier Catalog Number: orb1935497
• Alternative Catalog Number: BYT-ORB1935497-100,BYT-ORB1935497-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: CDHE, UVO

Product Description

E Cadherin (CDH1) Antibody (N-term)

Product Properties

• Clonality: Polyclonal
• Clone Designation: [RB13430]
• Molecular Weight: 97456 Da
• NCBI: 004351
• UniProt: P12830
• Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Target: This E Cadherin (CDH1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1).

Application Notes

• Application Dilute: WB - 1:2000, FC - 1:10-50

Images

Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-CDH1 Antibody (N-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: DU145 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lane 6: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.