Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Target:
SPART
Application Dilute:
WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
Western blot analysis of SPG20 Antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). SPG20 (arrow) was detected using the purified Pab.
SPG20 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with SPG20 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.
orb39730
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