Sirius Red Total Collagen Detection Kit

Catalog Number: CHX-9062
Article Name: Sirius Red Total Collagen Detection Kit
Biozol Catalog Number: CHX-9062
Supplier Catalog Number: 9062
Alternative Catalog Number: CHX-9062
Manufacturer: Chondrex
Category: Kits/Assays
Sirius Red Total Collagen Detection Kit

Sirius red is a unique dye which specifically binds to the [Gly-X-Y]n helical structure on fibrillar collagen (type I to V) and does not discriminate between collagen species and types.

Chondrex, Inc. provides a Sirius Red Total Collagen Detection Assay Kit for detecting the total collagen content in various collagen-containing samples such as tissue specimens, cell culture media, and cultured cells.

The total assay working time is less than 30 minutes and 40 samples can be measured in duplicate. Due to the low level of collagen in cell culture media, additional concentration steps may be necessary. For determining levels of collagen from individual species or different types of samples, Chondrex, Inc. recommends our Type I Collagen Detection ELISA kits and Type II Collagen Detection ELISA Kit. For convenience, the same sample preparations may be used for both the Collagen Detection ELISA Kits and the Sirius Red Total Collagen Detection Assay Kit.

 

Kit Components

  • 1 ml Standard - Bovine Type I Collagen (90621)
  • 50 ml Sirius Red Solution (90622)
  • 50 ml Washing Solution (90623)
  • 30 ml Extraction Solution (90624)
  • 20 ml 0.5M Acetic Acid (10X Acetic Acid) (90625)
  • 96-Well Plate (9026)
Range: 500 µg/ml-8 µg/ml
Storage: -20°C
Samples: Culture Media, Solubilized Collagen