Rekombinantes (HIS)6-p53-Protein

The mouse monoclonal anti-(His)6-tag antibody, clone 13/45/31-2 (H. Zentgraf/DKFZ Heidelberg, Germany), specifically detects any kind of histidine-tagged proteins in cells and complex cellular lysates. This monoclonal antibody specifically reacts with recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or internally. A higher number of histidine residues leads to an increased binding affinity of the antibody, e.g. an expression construct with 10 histidine residues increases the affinity about 10- to 20-fold. Additional flanking amino acids are not required for antibody binding, therefore enabling the choice of many different expression vectors on the only condition that the TAG-epitope is sterically available.

The Antibody clone 13/45/31-2 /13/45/31 is with more than 100 citations one of the most cited His-Tag antibodies world wide.

 

Background

Recombinant proteins are utilized for various purposes in molecular biology. Many prokaryotic expression vectors have been established enabling synthesis of the protein of interest as a fusion with a peptide, thus facilitating purification. Expression of recombinant proteins in E. coli as a fusion protein that includes histidine residues (His) as tag is one of the most popular methods, because histidine tagged proteins have useful attributes. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbant and the resulting protein can be selectively detected using antibodies such as clone 13/45/31-2.

 

Product Specifications

Artikelnummer	DIA-900-100
Spezifität	6-His Epitop-Tag, His Epitop-Tag, His-tag (N/C-terminal)
Spezies-Reaktivität	alle, rekombinant
Immunogen	Rekombinantes (HIS)6-p53-Protein
Wirtsspezies	Maus
Isotyp	IgG1
Klon	13/45/31
Klonalität (Mono-/Polyklonal)	monoklonal
Anwendung	Durchflusszytometrie (Flow Cytometry), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Western Blot
Konjugation	unkonjugiert
Verdünnung	Durchflusszytometrie 5 – 10 µl/10^6 Zellen, ELISA 0,1 – 0,4 µg/ml, Immunfluoreszenz 1 – 4 µg/ml, Immunhistochemie (IHC): 1 – 4 µg/ml, Immunpräzipitation 4 µg/ml, Western Blot (WB): 1 – 4 µg/ml
Format	0,05% NaN3, 6% BSA, in PBS (pH 7,4), ProteinA/G-gereinigter Antikörper (aus Kulturüberstand)
Anwendungshinweis	Achtung geänderte Konzentration: Jetzt 1 mg/ml!
Produktlinie / Thema	Epitop-Tags, Haptene und Label
Zweckbestimmung	nur für Forschungszwecke
Temperatur - Lagerung	2-8°C
Temperatur - Transport	bei Raumtemperatur
Suchcode	6his, anti his, DIA900, hexahistidine, his6, histag, histidin tag, histidine, polyhistidine
Hersteller / Marke	dianova
Uniprot_ID	N/A
Gene_ID	N/A
Bestellhinweis	Neue Produktnummer, ersetzt dia-900 (Neue Konzentration!)
Alias	6*His, 6His, 6histag, 6XHis, hexahistidine, HHHHHH, His Epitop-Tag, His Epitope-Tag, His Tag, his6, histidin tag, polyhistidine