Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice.
Conjugation:
Unconjugated
Alternative Names:
Human IgG F(c) Antibody, Human IgG Fc Antibody, Ms-a-Human IgG Fc, Human IgG F(c) Antibody in Mouse, Human Secondary Antibody
Format:
IgG1
Target Specificity:
IgG (Fc)
Cross-Adsorption (MinX):
no cross-adsorbtion
Anti-Human IgG F(c) generated in mouse detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of hum
Clonality:
Monoclonal
Concentration:
1.0 mg/ml
Clone Designation:
[3D8.H7.D4]
Isotype:
IgG1
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-Human IgG F(c) Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG, Human Serum and Human F(c). No reaction was observed against Human F(ab).
Form:
Liquid (sterile filtered)
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Human
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1-:1000-1:10000, Western Blot Dilution: 1:1000 - 1:2000
Application Notes:
Human IgG F(c) antibody is suitable for immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
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