Rat IgG anti-Rabbit IgG (H+L)-Alk. Phos., MinX Hu,Go,Ms
Biozol Catalog Number:
DNA-SEC-183408
Supplier Catalog Number:
SEC-183408
Alternative Catalog Number:
DNA-SEC-183408
Manufacturer:
dianova
Host:
Rat
Category:
Antikörper
Application:
ELISA,IHC,WB
Species Reactivity:
Rabbit
Immunogen:
Rabbit IgG whole molecule
Conjugation:
Alk. Phos.
Alternative Names:
Rat Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Rat Anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation
Format:
IgG
Target Specificity:
IgG (H+L)
Cross-Adsorption (MinX):
Human,Goat,Mouse
Anti-Rabbit IgG (H&L) generated in rat detects rabbit Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins. Specificity was confirmed using ELISA at less than 1% cross reactivity against the sources listed above.
Form:
Liquid (sterile filtered)
Formula:
50 mM TrisHCl,150 mM NaCl,1 mM MgCl,0,1 mM ZnCl,50% (v/v) Glycerol,pH 8,0,sterile filtered,0,01% NaN3
Target:
Rabbit
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:1,500 - 1:6,000, Immunohistochemistry Dilution: 1:200 - 1:1,000, Western Blot Dilution: 1:500 - 1:2,000
Application Notes:
Anti-Rabbit IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
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