Rat IgG anti-Rabbit IgG (H+L)-Biotin, MinX Hu,Go,Ms
Biozol Catalog Number:
DNA-SEC-183409
Supplier Catalog Number:
SEC-183409
Alternative Catalog Number:
DNA-SEC-183409
Manufacturer:
dianova
Host:
Rat
Category:
Antikörper
Application:
ELISA,IHC,WB
Species Reactivity:
Rabbit
Immunogen:
Rabbit IgG whole molecule
Conjugation:
Biotin
Alternative Names:
Rat Anti-Rabbit IgG Biotin Conjugated Antibody, Rat Anti-Rabbit IgG Antibody Biotin Conjugation
Format:
IgG
Target Specificity:
IgG (H+L)
Cross-Adsorption (MinX):
Human,Goat,Mouse
Anti-Rabbit IgG (H&L) generated in rat detects rabbit Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human, Goat and Mouse Serum Proteins.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target:
Rabbit
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:52,000 is suggested for this product.
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