NFkB p65 Mouse mAb, IgG1, Monoclonal

Product Overview

• Article Name: NFkB p65 Mouse mAb, IgG1, Monoclonal
• Biozol Catalog Number: EBT-EM1111
• Supplier Catalog Number: EM1111
• Alternative Catalog Number: EBT-EM1111-50, EBT-EM1111-100
• Manufacturer: ELK Biotechnology
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant Protein
• Alternative Names: NFKB3, p65, RELA, Transcription factor p65

Product Description

NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/mL
• Molecular Weight: 65kDa
• Isotype: IgG1
• NCBI: 5970
• UniProt: Q04206

Application Notes

• Application Dilute: WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500

Images

Western blot analysis ofextracts from HeLa (Lane 1),MCF-7 (Lane 2), Mouse Liver (Lane 3), Rat Kidney (Lane 4), using NFkappaB p65 dilutedat1:1,500.

1,Input: Hela Cell Lysate 2,IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1111 1:2,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000

IF analysis of Hela with EM1111(Left) and DAPI (Right) diluted at 1:100.

Immunohistochemical analysis of paraffin-embeddedRat Brain Tissue using NFkB p65 (EM1111)Mouse mAbdiluted at 1:500.