This 4-Color Multiple Fluorescent Staining Kit (mIHC) is suitable for multiplex immunofluorescence staining of tissue samples, including paraffin sections, frozen sections, cell slides, cell smears, and organoids. It is particularly suitable for multiplex fluorescent immunolabeling using primary antibodies from the same source, but can also be used with antibodies from different sources. The primary principle of TSA is based on tyramide signal amplification (TSA), hereinafter referred to as TSA technology. TSA utilizes the peroxidase reaction of tyramide (wherein a fluorescently labeled tyramide forms a covalent binding site under the catalysis of HRP and H2O2). This generates a large number of enzymatic reactions, which then bind to surrounding protein residues (including tryptophan, histidine, and tyrosine residues), forming a large amount of fluorescein deposited at the antigen-antibody binding site, thereby amplifying the signal. This kit uses multiple fluorescent dyes to label tyramide, resulting in strong and stable fluorescence, making it suitable for repeated immunolabeling and multiplex fluorescent staining.
RA10009
RA10009
RA10009
RA10009
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