Product information

The CellTram 4r Air is a manual microinjector suitable for a broad range of life science applications. The CellTram 4r Air is a pneumatic injector for oil-free micromanipulation and gentle holding of cells in suspension. Due to its excellent performance, it is also highly suitable for uptake and injection of cells (e.g., of sperm or ES cells). For this application, its performance is comparable to that of oil-filled injectors. The model can be used with all common micromanipulation systems yet it develops its full potential with Calibre Scientific manipulators. The CellTram 4r Air injector is for research use only.

 

Applications

• CSI of mouse and large mammals (e.g., equine, porcine...)
• Holding of sensitive oocytes, embryos or small organisms
• Piezo-assisted micromanipulation in combination with the Calibre Scientific PiezoXpert®
• Transfer of cells or organelles (e.g., ES cell or iPS cell transfer into blastocysts or morulae)
• Enucleation/nuclear transfer
• (Xeno)transplantations
• Removal of cells (e.g., for polar body biopsy, blastomere biopsy or trophectoderm biopsy)
• Manual microinjection and dispensing of aqueous solutions
• Single cell picking

 

Features

• Non-hydraulic system for oil-free manipulation and injection of cells
• Piston position scale enables setting of air volume and injection characteristics according to individual needs
• Coarse and fine drive for precise and intuitive sample control
• Improved rotary knob design for better handling and easier cleaning
• Capillary holder 4 with markings for easier positioning
• New grip head 4 system for simple capillary change and higher user safety
• Position indicators and marking for in/out to reduce risk of user errors
• Stable, nonslip base plate
• Highly reliable performance with minimal maintenance
• Oil-free system with optimized resolution for holding larger cells in suspension and injection of cells (e.g., sperm)
• Simple setting of operating point via pressure compensation
• Extremely simple operation because no oil filling is required
• Two rotary knobs of different transmission ratio (1:1 and 1:10) for the highest standards in terms of resolution, sensitivity and response behaviour
• Compatible with most commonly used micromanipulators

 

Technical data

Specifications	CellTram 4r Air
Injection tube	Fluorinated ethylene propylene (FEP), 1.3 m length, inner diameter 0.5 mm, outer diameter 2 mm
Pressure generation	Cylinder/piston system, air-filled
Volume change per revolution	60 µL/600 µL (fine / coarse rotary knob)
Cylinder volume	10 mL
Min. adjustable volume	< 100 nL
Max. pressure	3000 hPa