CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms - FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[MEM-154]
Isotype:
Mouse IgG1
Buffer:
Phosphate buffered saline (PBS), pH 7.4
Storage:
2°C to 8°C
Target:
CD16
Antibody Type:
Monoclonal Antibody
Application Notes:
Functional application: The antibody MEM-154 blocks binding of human IgG to FcgammaRIII. Flow cytometry: Recommended dilution: 5-10 µg/ml, positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Western blotting: Non-reducing conditions.
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