WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX113486 ELISA Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE GTX113486 diluted at 1:10000
Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Galectin3 antibody detects Galectin3 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: Galectin3 protein stained by Galectin3 antibody (GTX113486) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Galectin3 antibody detects Galectin3 protein by western blot analysis. A. 50 μg rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody (GTX113486) dilution: 1:10000
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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