Dengue virus NS3 protein antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
GTX124252
- Images (7)
| Article Name: | Dengue virus NS3 protein antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | GTX124252 |
| Supplier Catalog Number: | GTX124252 |
| Alternative Catalog Number: | GTX124252-100,GTX124252-25 |
| Manufacturer: | GeneTex |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FACS, ICC, IHC, IHC-P, IP, WB |
| Species Reactivity: | Virus |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary. |
| Conjugation: | Unconjugated |
| Alternative Names: | Dengue Virus Non-structural protein 3 , Dengue Virus NS3 , DENV Non-structural protein 3 , DENV NS3 |
| Clonality: | Polyclonal |
| Concentration: | 0.15 mg/ml (Please refer to the vial label for the specific concentration.) |
| Molecular Weight: | 70 |
| Sensitivity: | Based on internal testing results, this antibody may cross react with Dengue virus type 1/3/4. |
| Buffer: | 1XPBS pH7, 1% BSA, 20% Glycerol, 0.025% ProClin 300. |
| Purity: | Purified by antigen-affinity chromatography. |
| Form: | Liquid |
| Application Notes: | WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications. |
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GTX124252 ICC/IF Image |
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NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000. Blue: Hoechst 33342 staining. |
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NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000. Blue: Hoechst 33342 staining. |
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Sample (20 μg of whole cell lysate) A: BHK-21 B: Dengue virus 2 infect BHK-21 C: JEV infect BHK-21 7.5% SDS PAGE GTX124252 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis. A. 30 μg BHK-21 whole cell extract B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells 7.5% SDS-PAGE NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000. Blue: Hoechst 33342 staining. |
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Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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