Dengue virus Envelope protein antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Dengue virus Envelope protein antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX127277
• Supplier Catalog Number: GTX127277
• Alternative Catalog Number: GTX127277-100,GTX127277-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IHC-P, WB
• Species Reactivity: Virus
• Immunogen: Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: Dengue Virus Envelope , Dengue Virus E , DENV Envelope , DENV E

Product Properties

• Clonality: Polyclonal
• Concentration: 1.33 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 54
• Sensitivity: This antibody was raised against Dengue virus 2 Envelope protein, which may cross react with Envelope protein of Dengue virus 3 but does not recignize with Envelope protein of JEV.
• Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX127277 ICC/IF Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infected BHK-21
C: JEV infected BHK-21
12% SDS PAGE
GTX127277 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein by western blot analysis. Un-infected (-) and infected (+, Dengue virus 2 infection) BHK-21 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody (GTX127277) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min