Zika virus Envelope protein antibody [GT363], IgG2b, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: Zika virus Envelope protein antibody [GT363], IgG2b, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX634155
• Supplier Catalog Number: GTX634155
• Alternative Catalog Number: GTX634155-100,GTX634155-25
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, IHC, WB
• Species Reactivity: Virus
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: Zika virus Envelope , ZIKV Envelope

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [GT363]
• Molecular Weight: 54
• Isotype: IgG2b
• Buffer: PBS, no Preservative.
• Purity: Affinity purified by Protein A.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:50-1:3000. ICC/IF: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX634155 ICC/IF Image

Non-infected (–) and infected (+) Vero cell extracts (15 μg, unboiled) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) culture supernatant diluted at 1:50.

Sandwich ELISA detection of Zika virus-infected lysate using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133326 as capture antibody at concentration of 5 μg/mL and GTX634155 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus Envelope protein antibody [GT363] (GTX634155).
Green: Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:500.

Untreated (–) and treated (+) C6/36 cell extracts (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:2000.