PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68], Unconjugated

Article Name:	PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68], Unconjugated
Biozol Catalog Number:	HBO-ET1608-56
Supplier Catalog Number:	ET1608-56
Alternative Catalog Number:	HBO-ET1608-56
Manufacturer:	HUABIO
Host:	Rabbit
Category:	Antikörper
Application:	FC, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Synthetic peptide within N-terminal human PARP1.
Conjugation:	Unconjugated
Alternative Names:	ADP ribosyltransferase (NAD+, poly (ADP ribose) polymerase) antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD(+) ADP

The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once P

TARGET

UNIPROT #P09874

PROTEIN NAME PARP

SYNONYMS ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody; ADP ribosyltransferase diphtheria toxin like 1 antibody; ADP ribosyltransferase NAD(+) antibody; ADPRT 1 antibody; ADPRT antibody; ADPRT1 antibody; ARTD1 antibody; msPARP antibody; NAD(+) ADP ribosyltransferase 1 antibody; NAD(+) ADP-ribosyltransferase 1 antibody; pADPRT 1 antibody; pADPRT1 antibody; PARP 1 antibody; PARP antibody; PARP-1 antibody; PARP1 antibody; PARP1_HUMAN antibody; Poly (ADP ribose) polymerase 1 antibody; poly (ADP ribose) polymerase family, member 1 antibody; Poly [ADP-ribose] polymerase 1 antibody; Poly(ADP ribose) polymerase antibody; poly(ADP ribose) synthetase antibody; poly(ADP ribosyl)transferase antibody; Poly[ADP ribose] synthetase 1 antibody; Poly[ADP-ribose] synthase 1 antibody; PPOL antibody

POST-TRANSLATIONAL MODIFICATION Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.

SUBCELLULAR LOCATION Nucleus.

FUNCTION PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

Clonality:	Monoclonal
Concentration:	1 mg/mL.
Clone Designation:	[SU03-68]
Molecular Weight:	Predicted band size: 113 kDa
Isotype:	IgG
UniProt:	P09874
Buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purity:	Protein A affinity purified.
Form:	Liquid
Target:	PARP-1, ARTD1, ADPRT 1, PARP1
Antibody Type:	Primary Antibody

Application Dilute:

WB: 1:2,000 ,IF-Cell: 1:500 ,IF-Tissue: 1:50-1:800 ,IHC-P: 1:500-1:4,000 ,FC: 1:1,000

	Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.