Anti-ATG5 Antibody [SN73-07], Unconjugated, Rabbit, Monoclonal

Product Overview

• Article Name: Anti-ATG5 Antibody [SN73-07], Unconjugated, Rabbit, Monoclonal
• Biozol Catalog Number: HBO-ET1611-38
• Supplier Catalog Number: ET1611-38
• Alternative Catalog Number: HBO-ET1611-38
• Manufacturer: HUABIO
• Host: Rabbit
• Category: Antikörper
• Application: IHC-P, IP, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: Synthetic peptide within Human ATG5 aa 1-50 / 275.
• Conjugation: Unconjugated
• Alternative Names: APG 5 antibody APG 5L antibody APG5 antibody APG5 autophagy 5 like antibody APG5 like antibody APG5-like antibody APG5L antibody Apoptosis specific protein antibody Apoptosis-specific protein antibody ASP antibody ATG 5 antibody Atg5 antibody ATG5 autoph

Product Description

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjuga

Additional Information

TARGET

UNIPROT #Q9H1Y0

PROTEIN NAME ATG5

SYNONYMS APG 5 antibody; APG 5L antibody; APG5 antibody; APG5 autophagy 5 like antibody; APG5 like antibody; APG5-like antibody; APG5L antibody; Apoptosis specific protein antibody; Apoptosis-specific protein antibody; ASP antibody; ATG 5 antibody; Atg5 antibody; ATG5 autophagy related 5 homolog antibody; ATG5_HUMAN antibody; Autophagy protein 5 antibody; Autophagy related 5 antibody; hAPG5 antibody; Homolog of S Cerevisiae autophagy 5 antibody; OTTHUMP00000040507 antibody

SEQUENCE SIMILARITIES Belongs to the ATG5 family.

TISSUE SPECIFICITY Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

POST-TRANSLATIONAL MODIFICATION Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.; Acetylated by EP300.

SUBCELLULAR LOCATION Cytoplasm, Preautophagosomal structure membrane.

FUNCTION Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL.
• Clone Designation: [SN73-07]
• Molecular Weight: Predicted band size: 32 kDa
• Isotype: IgG
• UniProt: Q9H1Y0
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Purity: Protein A affinity purified.
• Form: Liquid
• Target: ATG5
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1,000-1:5,000 ,IF-Cell: 1:50-1:200 ,IF-Tissue: 1:50-1:200 ,IHC-P: 1:50-1:100 ,IP: Use at an assay dependent concentration.

Images

ICC staining of ATG5 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).