Magic Red(TM) Cathepsin B Assay Kit
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| Article Name: | Magic Red(TM) Cathepsin B Assay Kit |
| Biozol Catalog Number: | ICT-937 |
| Supplier Catalog Number: | 937 |
| Alternative Catalog Number: | ICT-937 |
| Manufacturer: | ImmunoChemistry Technologies |
| Category: | Kits/Assays |
Quantitate and monitor intracellular cathepsin-B activity over time in vitro. The Magic Red substrate in this assay fluoresces red upon cleavage by active cathepsin enzymes. Analyze the fluorescent signal using fluorescence microscopy or a fluorescence plate reader.
Background & Details Elevated cathepsin enzyme activity in serum or the extracellular matrix can signify a number of pathological conditions. ICT’s Magic Red assay kits are used by researchers seeking to quantitate and monitor cathepsin activity in cultured cells and tissues. This assay kit detects cathepsin-B activity. To use Magic Red, add the substrate directly to the cell culture media, incubate, and analyze. Because it is cell permeant, it easily penetrates the cell membrane and the membranes of the internal cellular organelles – no lysis or permeabilization steps are required. If cathepsin enzymes are active, the Magic Red substrate is cleaved and the cresyl violet fluorophore will become fluorescent upon excitation. As enzyme activity progresses and more Magic Red substrate is cleaved, the signal will intensify, allowing researchers to watch the color develop over time. Samples can be analyzed by fluorescence microscopy or with a fluorescence plate reader. Hoechst 33342 and Acridine Orange are included in the kit to detect nuclear morphology and lysosomal organelle structure, respectively.
Target Cathepsin B Excitation / Emission 592 nm / 628 nm Method of Analysis Fluorescence Plate Reader, Fluorescence Microscope
Kit Contents ICT-937 (25 Tests) |
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Magic Red® Cathepsin-B Assay Kit |
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Magic Red® Cathepsin-B Assay Kit (Components) |
