Non-biotinylated Human ACE2/ACEH Protein (ACE-HM401)

Product Overview

• Article Name: Non-biotinylated Human ACE2/ACEH Protein (ACE-HM401)
• Biozol Catalog Number: KAB-ACE-HM401
• Supplier Catalog Number: ACE-HM401
• Alternative Catalog Number: KAB-ACE-HM401-100UG, KAB-ACE-HM401-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: ACE-2, ACEH, ACE2

Product Description

Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.

Product Properties

• Molecular Weight: The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q9BYF1
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Gln18-Ser740
• Target: ACE2/ACEH

Images

Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC

SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).

Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).

Loaded SARS-COV-2 Spike S1, hFc Tag on ProA-Biosensor can bind Non-biotinylated Human ACE2, His Tag with an affinity constant of 0.66 nM as determined in BLI assay (Gator Prime).