Recombinant Human/Mouse/Rat Activin A Protein is expressed from HEK293 without tag.It contains Gly311-Ser426.
Molecular Weight:
The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE
Form:
Lyophilized
Sequence:
Gly311-Ser426
Target:
Activin A
Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).
Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).
Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human/Mouse/Rat Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).
Human ACVR1B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 18.43 nM as determined in SPR assay (Biacore T200).
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