Human ALCAM/CD166 Protein (ALC-HM201)

Product Overview

• Article Name: Human ALCAM/CD166 Protein (ALC-HM201)
• Biozol Catalog Number: KAB-ALC-HM201
• Supplier Catalog Number: ALC-HM201
• Alternative Catalog Number: KAB-ALC-HM201-100UG, KAB-ALC-HM201-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD166, ALCAM, MEMD, CD6L, KG-CAM

Product Description

Recombinant Human ALCAM/CD166 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Trp28-Ala526.

Product Properties

• Molecular Weight: The protein has a predicted MW of 82.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: Q13740
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Trp28-Ala526
• Target: ALCAM/CD166

Images

Human ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC.

Immobilized Human ALCAM, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA.

Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).

Immobilized Human CD6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test).