Recombinant Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus).
Molecular Weight:
The protein has a predicted MW of 16.56 kDa. Due to glycosylation, the protein migrates to 33-43 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)
Target:
Activin RIIB/ACVR2B
The purity of Biotinylated Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.
Biotinylated Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human Activin A, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 53.8ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human/Cynomolgus Activin RIIB, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Activin RIIB Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Biotinylated Human/Cynomolgus Activin RIIB, His Tag immobilized on CM5 Chip can bind Anti-Activin RIIB Antibody, hFc Tag with an affinity constant of 6.44 pM as determined in SPR assay (Biacore T200).
Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).
Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
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