Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112)

Product Overview

• Article Name: Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112)
• Biozol Catalog Number: KAB-BAF-HM112
• Supplier Catalog Number: BAF-HM112
• Alternative Catalog Number: KAB-BAF-HM112-100UG, KAB-BAF-HM112-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: BAFF, BLyS, CD257, TNFSF13B, TNFSF20, DTL, TALL1, TALL-1delta BAFF, TALL1Delta4 BAFF, THANK, ZTNF4, TALL-1

Product Description

Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Thr141-Leu285 trimer design.

Product Properties

• Molecular Weight: The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
• Tag: N-His-Flag
• UniProt: Q9Y275
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Thr141-Leu285
• Target: BAFF/TNFSF13B/CD257

Images

Human BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC.

Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200).

Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test).

Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.

Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 1.53 nM as determined in BLI assay (Gator Prime).

Loaded Biotinylated Human BAFFR, His-Avi Tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator Prime).

Immobilized Human BAFF Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BAFF Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.