Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala134-Leu285.
Molecular Weight:
The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-25 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ala134-Leu285
Target:
BAFF/TNFSF13B/CD257
Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC.
Immobilized Human BAFFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
Immobilized Human BCMA, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1ng/ml determined by ELISA.
Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay (Gator Prime).
The purity of Biotinylated Human BAFF is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS.
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