Non-biotinylated Mouse BCMA/TNFRSF17 Protein (BCM-MM417)

Product Overview

• Article Name: Non-biotinylated Mouse BCMA/TNFRSF17 Protein (BCM-MM417)
• Biozol Catalog Number: KAB-BCM-MM417
• Supplier Catalog Number: BCM-MM417
• Alternative Catalog Number: KAB-BCM-MM417-100UG, KAB-BCM-MM417-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Mouse
• Alternative Names: CD269, TNFRSF17, BCMA, BCM, TNFRSF13A

Product Description

Recombinant Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Thr49.

Product Properties

• Molecular Weight: The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: O88472
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE
• Form: Lyophilized
• Sequence: Met1-Thr49
• Target: BCMA/TNFRSF17

Images

Mouse BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).

Immobilized Mouse BCMA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test).

Immobilized Mouse BCMA, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Mouse BAFF, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.

Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 39.20 nM as determined in BLI assay (Gator Prime).

Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse APRIL, hFc Tag with an affinity constant of 7.09 nM as determined in BLI assay (Gator Prime).