Biotinylated Human CD19 Protein (CD1-HMR102)

Product Overview

• Article Name: Biotinylated Human CD19 Protein (CD1-HMR102)
• Biozol Catalog Number: KAB-CD1-HMR102
• Supplier Catalog Number: CD1-HMR102
• Alternative Catalog Number: KAB-CD1-HMR102-100UG, KAB-CD1-HMR102-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: CD19, B4, CVID3, Leu-12, MGC12802

Product Description

Recombinant Biotinylated Human CD19 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Pro20-Lys291.

Product Properties

• Molecular Weight: The protein has a predicted MW of 36 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P15391
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE
• Form: Lyophilized
• Sequence: Pro20-Lys291
• Target: CD19

Images

Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA.

Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).

Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).

Loaded Coltuximab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of 4.53 nM as determined in BLI assay (Gator Prime).

Loaded Denintuzumab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of4.70 nM as determined in BLI assay (Gator Prime).

Loaded Inebilizumab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of 11.00 nM as determined in BLI assay (Gator Prime).