Human EGF Protein (EGF-HM202)

Product Overview

• Article Name: Human EGF Protein (EGF-HM202)
• Biozol Catalog Number: KAB-EGF-HM202
• Supplier Catalog Number: EGF-HM202
• Alternative Catalog Number: KAB-EGF-HM202-100UG, KAB-EGF-HM202-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: HOMG4, EGF, URG

Product Description

Recombinant Human EGF Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Asn971-Arg1023.

Product Properties

• Molecular Weight: The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result.
• Tag: N-hFc (IgG1)
• UniProt: P01133
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Asn971-Arg1023
• Target: EGF

Images

Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human EGF is greater than 95% as determined by SEC-HPLC.

Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200).

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50-for this effect is typically 0.2 - 1.5 ng/mL.

Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).

Loaded Human EGF, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator Prime).