Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling) (FSF-HM416B)
Biozol Catalog Number:
KAB-FSF-HM416B
Supplier Catalog Number:
FSF-HM416B
Alternative Catalog Number:
KAB-FSF-HM416B-100UG, KAB-FSF-HM416B-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Biotin
Alternative Names:
TL1A, VEGI-251, TNFSF15, TL1, VEGI, VEGI192A
Recombinant Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Asp91-Leu251 trimer design.
Molecular Weight:
The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Asp91-Leu251
Target:
TNFSF15
The purity of Biotinylated Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TNFSF15 Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Mouse DR3, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.22 ug/ml determined by ELISA (QC Test).
Immobilized Human DR3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.34ug/ml determined by ELISA.
Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human TNFSF15 Trimer, His Tag with an affinity constant of 3.64 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human TNFSF15 Trimer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DcR3, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA.
Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Biotinylated Human TNFSF15 (Trimer), His Tag with an affinity constant of 24.60 nM as determined in BLI assay (Gator).
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