Non-biotinylated Mouse TNFSF15 Protein (active trimer) (FSF-MM415)

Product Overview

• Article Name: Non-biotinylated Mouse TNFSF15 Protein (active trimer) (FSF-MM415)
• Biozol Catalog Number: KAB-FSF-MM415
• Supplier Catalog Number: FSF-MM415
• Alternative Catalog Number: KAB-FSF-MM415-100UG, KAB-FSF-MM415-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Mouse
• Alternative Names: TL1A, VEGI-251, TNFSF15, TL1, VEGI, VEGI192A

Product Description

Recombinant Mouse TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala61-Leu252.

Product Properties

• Molecular Weight: The protein has a predicted MW of 24.40 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
• Tag: N-His-Avi
• UniProt: Q5UBV8
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala61-Leu252
• Target: TNFSF15

Images

Mouse TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse TNFSF15 is greater than 95% as determined by SEC-HPLC.

Mouse DR3, His Tag immobilized on CM5 Chip can bind Mouse TNFSF15, His Tag with an affinity constant of 0.96 nM as determined in SPR assay (Biacore T200).

Immobilized Mouse TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).

Immobilized Mouse TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA.

The purity of Mouse TNFSF15 is greater than 95% and the molecular weight of this protein is around 70-95 kDa as determined by SEC-MALS.