Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu72-Leu251
Target:
TNFSF15
Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA.
The purity of Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.
Human TNFSF15 Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Loaded Anti-TNFSF15 Antibody, hFc Tag on ProA-Biosensor can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.27 nM as determined in BLI assay (Gator Prime).
Loaded Human DR3, hFc Tag on ProA-Biosensor can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.23 nM as determined in BLI assay (Gator Prime).
Immobilized Human TNFSF15 Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 20.6ng/ml determined by ELISA.
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