Lyophilized from 0.22 µm filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE
Form:
Lyophilized
Sequence:
Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat)
Target:
GDF-8
Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%.
Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).
Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA.
Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.
Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test).
Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Biotinylated Activin RIIB, His Tag-captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
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