Biotinylated Human/Mouse/Rat GDF-8 Protein (GDF-HM008B)

Product Overview

• Article Name: Biotinylated Human/Mouse/Rat GDF-8 Protein (GDF-HM008B)
• Biozol Catalog Number: KAB-GDF-HM008B
• Supplier Catalog Number: GDF-HM008B
• Alternative Catalog Number: KAB-GDF-HM008B-100UG, KAB-GDF-HM008B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Biotin
• Alternative Names: Myostatin, GDF8, Mstn, GDF-8, Myostatin

Product Description

Recombinant Biotinylated Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 with Avi tag at the C-terminus.It contains Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat).

Product Properties

• Molecular Weight: The protein has a predicted MW of 14.21 kDa. Due to glycosylation, the protein migrates to 15-18 kDa based on Bis-Tris PAGE result.
• Tag: C-Avi
• UniProt: O14793
• Buffer: Supplied as 0.22 µm filtered solution in 4mM HCL.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE
• Form: Liquid
• Sequence: Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat)
• Target: GDF-8

Images

Biotinylated Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human/Cynomolgus Activin RIIA, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat GDF-8, Avi Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test).

Immobilized Biotinylated Human/Mouse/Rat GDF-8, Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA.

Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat GDF-8, Avi Tag with the EC50 of 2.5ng/ml determined by ELISA.

Loaded Human/Cynomolgus Activin RIIB, hFc Tag on ProA-Biosensor can bind Biotinylated Human/Mouse/Rat GDF-8, His Tag with an affinity constant of 0.22 nM as determined in BLI assay (Gator Prime).

Loaded Anti-GDF8 Ab., hFc Tag on ProA-Biosensor can bind Biotinylated Human/Mouse/Rat GDF-8, His Tag with an affinity constant of 0.49 nM as determined in BLI assay (Gator Prime).