Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-HM41CT)

Product Overview

• Article Name: Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-HM41CT)
• Biozol Catalog Number: KAB-HLG-HM41CT
• Supplier Catalog Number: HLG-HM41CT
• Alternative Catalog Number: KAB-HLG-HM41CT-100UG, KAB-HLG-HM41CT-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: HLA G antigen, sHLA-G, b2 microglobulin, HLA G, HLAG, HLA-G, MHC Class I Antigen G, MHC class Ib antigen, MHC-G, sHLA-G

Product Description

Recombinant Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide.

Product Properties

• Molecular Weight: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P17693
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide
• Target: HLA-G&B2M&Peptide (RIIPRHLQL)

Images

Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.

The purity of Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer is greater than 90% as determined by SEC-HPLC.

Serial dilutions of Anti-LILRB2 Antibody were added into Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.11ug/ml.

Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer with an affinity constant of 4.62 nM as determined in SPR assay (Biacore T200).

Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5uM as determined in a SPR assay (Biacore T200).

Immobilized Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).