Human IL-18 Protein (IL1-HE018)

Product Overview

• Article Name: Human IL-18 Protein (IL1-HE018)
• Biozol Catalog Number: KAB-IL1-HE018
• Supplier Catalog Number: IL1-HE018
• Alternative Catalog Number: KAB-IL1-HE018-100UG, KAB-IL1-HE018-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IL-18, IL18, IGIF, IL-1F4, IL-1 gamma, IL1F4, IL-1g, Interleukin 1, IL-1gamma, IL-1 gamma

Product Description

Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193.

Product Properties

• Molecular Weight: The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: Q14116
• Buffer: Lyophilized from 0.22µm filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Tyr37-Asp193
• Target: IL-18

Images

Human IL-18 R1, His Tag (Cat. IL8-HM4R1) captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).

Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC.

Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag (Cat. IL8-HM2BP) with the EC50 of 62.0ng/ml determined by ELISA (QC Test).

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 1-10 ng/mL (QC Test).

Loaded Human IL-18BP, His Tag (Cat. IL8-HM1BP) on Anti-His-Biosensor can bind Human IL-18, No Tag with an affinity constant of 1.80 nM as determined in BLI assay (Gator Prime).