Recombinant Human IL-13 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Gly21-Asn132.
Molecular Weight:
The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Tag:
N-hFc (IgG1)
UniProt:
AAK53823
Buffer:
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly21-Asn132
Target:
IL-13
Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC.
Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Immobilized Human IL-13, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 7.1 ng/ml determined by ELISA.
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test).
Loaded Human IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra1, His Tag with an affinity constant of 1.15 µM as determined in BLI assay (Gator Prime).
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