Human IL-2 Protein (IL2-HE001)

Product Overview

• Article Name: Human IL-2 Protein (IL2-HE001)
• Biozol Catalog Number: KAB-IL2-HE001
• Supplier Catalog Number: IL2-HE001
• Alternative Catalog Number: KAB-IL2-HE001-100UG, KAB-IL2-HE001-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IL2, T-cell growth factor, TCGF, Aldesleukin

Product Description

Recombinant Human IL-2 Protein is expressed from E.coli without tag.It contains Ala21-Thr153.

Product Properties

• Molecular Weight: The protein has a predicted 15.5 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P60568
• Buffer: Lyophilized from 0.22µm filtered solution in 100mM Acetic Acid. Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala21-Thr153
• Target: IL-2

Images

Human IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-2 is greater than 95% as determined by SEC-HPLC.

Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).

Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.25uM as determined in SPR assay (Biacore T200).

Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200).

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.5-2 ng/mL (QC Test).