Human IL-6 Protein (IL6-HE001)

Product Overview

• Article Name: Human IL-6 Protein (IL6-HE001)
• Biozol Catalog Number: KAB-IL6-HE001
• Supplier Catalog Number: IL6-HE001
• Alternative Catalog Number: KAB-IL6-HE001-100UG, KAB-IL6-HE001-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IFN-beta-2, IL6, IL-6, BSF-2, CDF, MGI-2A, Interleukin-6, HSF, IFNB2, HGF,IFN-beta-2

Product Description

Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212.

Product Properties

• Molecular Weight: The protein has a predicted 20.8 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P05231
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Val30-Met212
• Target: IL-6

Images

Human IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC.

Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.8 ng/mL.

Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA.

Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay (Gator Prime).